I will be really grateful if you could recommend which program is most suitable for our application.
I thought about “CPcharm" would be best for our purpose below but I am open to any suggestion.
Also I am wondering if I need to download earlier version of Cellprofiler (v2) to use “CPChram”.
I am also worried that having both Cell profiler (V4) and V2 on my computer would cause any problem.
Our aim is to classify chromosome images of wild type and various mutant cells (vertebrate cell line) synchronously entering mitosis from G2 phase.
Basically we have set of DAPI stained image sets at G2, 2.5 min from G2, 5 min from G2, 7.5 min from G2, 10min, 15 min, 30 min.
5 min: most cells are prophase
7.5 min: mix of prophase and very early pro metaphase
10 min: early pro metaphase
15 min: mid pro metaphase
30 min: late promeatpahse, metaphase
But we want to quantify the progression of individual data set and compare wild type and mutant cells at individual data point.
This is not high-throurghput images. Each data set would contain max 50 images.
We do not really need to measure the length/width of chromosomes. We just want to classify the morphologies.
Thanks you very much for your advice in advance.