I’m trying to calculate the area of the bubbles present in an image. The first image is the one I am currently using.
By applying the following Maco, I am getting the results obtained in the second image.
setThreshold(0, 254); run("Create Mask"); run("Analyze Particles...", "exclude clear add"); close(); resetThreshold(); roiManager("Show All");
You can see that not all the bubbles are captured, especially those that are very long (having a large area).
Any suggestions to solve this problem? Thanks.
Something that I want to do but I couldn’t figure out how to do it is:
- fill the bubbles in white color.
- leave the background in black.
- use Shape Descriptor Maps to get the distribution.