I am trying to perform the inter-trabecular angle (ITA) analysis on a trabecular VOI. The skeleton which displays at the end looks quite suitable, however the I-T angles seem to be much too small.

My defined parameters are:

Min valence 3

Max valence 5

Min trab length 5px

Margin 30px

Iterate pruning no

Use clusters yes

Is there something I should be changing in the parameters to get more realistic angles?

Shouldn’t the angles between a node with 3 connections be around 120 degrees if the 3 angles should add up to 360?