Intensity of wells Imaging quantification

I am looking to use either image j or cell profiler to one pick out each of the orange wells depicted below and be able quantify the relative intensities to be able to calculate reflectance/absorbance values of the tif image below. Any suggestions of which tool I should use and what imaging processing modules I should use? I would prefer to have it automatically pick out the wells that are shown rather than manually select /crop a circle for each of them. Thanks
bead_outlet001.nd2 - C=0.tif (4.0 MB)

Can you check your picture? I don’t see any well…

M.

Uploading: 2020_03_13_xxt_1001 (1).tif…