Intensity of object


For my project, I want to extract from my images:
1- all of objects in pixel and
2- obtain the intensity of the fluorescence + mean fluorescence by object

Can anybody help me?
Thanks a lot,


generally yes,
but it will be impossible for anybody here help you if you won’t provide us some example images and which are the object you are searching in those images…

I want to detect the spot and the intensity and see difference between different treatment.