I’m trying to find the best way to estimate the total intensity of (Gaussian-shaped) spots in 2D and 3D images in ImageJ.
There is GaussFit OnSpot, but it is 2D only (still it might be interesting how it compares to the other options).
TrackMate has a
SpotIntensityAnalyzer, but it simply sums up the pixels in a
SpotNeighborhood, which might be too inaccurate for our use case, and I’d favor accuracy over performance in this case (if someone already did some benchmarking on this, I’d actually be happy to see it).
I noticed there is
legacy-imglib(that appears to be used in @StephanPreibisch’s RadialSymmetryLocalization plugin) and I will check it out. Is there a reason why this is not yet in
imglib2, and are there plans to migrate or replace it??
Or is there some implementation in Image Ops that I missed?