I am trying to measure the movement of fluorescence in cells over a time lapsed experiment. In my pipeline I first use a mask to remove saturated cells. I then identify the cells of interest which have a fluorescent membrane that moves into the cytoplasm over time. I am trying to find a way to measure the intensity of the cytoplasm over the time lapsed experiment but I can’t seem to find a way to reliably identify objects that change in fluorescence over time.
I have attached two sample images which show the movement of the fluorescence and my pipeline.
pipeline.cpproj (582 KB)