I reconstructed a neuron in FIJI and ran the Sholl Analysis by selecting the ctrl+shift+A function in the middle of the soma. However, at a radius of 0, the output file displays there are 2 crossings.
Am I reconstructing the soma incorrectly? I create 2 points on the edges of the soma to create a separate path for the soma. I use the ctrl+shift+A functon in the middle of this path,using the crtl function to navigate this path. I viewed the FIJI tutorial, where the origin of the path is the middle of the soma, and the sample image also displayed 2 intersections at a radius of 0.
Thank you for your help!