I am relatively new to ImageJ and image analysis in general. After lots of reading on forums I managed to split and binarize two channels of a fluorescent microscopy image. My goal was to see whether the pixels from these two channels have similar spatial distribution. So I decided to use the image calculator and divine channel 1/channel 2 and then channel 2/channel 1. The results were showing as 32 bit float images. My hypothesis was that if both channels have similar pixel distribution I should be able to see that the area occupied by pixels with value of is comparable in both cases. So in order to do that I checked the area fraction box in the set measurements window, which according to imageJ manual should give me the % of area that pixels with values other than zero occupy. My question to you is the following. I know that NaNs are not calculated in the area fraction but what about the infinity values? Are these part of the final area fraction?