These issues exist when I updated imageJ to the latest version.
When I open an nd2 images with bio-formats, different from the situations in the older version, I have to change the colour mode from default to composed/colourized, or I will get a totally grey stack.
However, even when I choose the composed/colorized color mode, the channel colour seems quite different from what I have set when photographing. For example, the nd2 image consists of 4 channels: 1. FITC, 2. TRITC, 3. DAPI, 4. Cy5. But the colours displayed in ImageJ always becomes 1.green, 2. red, 3. blue, 4. RED as followed(which is supposed to be MAGENTA and works well in the older version). The same in all images no matter what channels are used and what the channel order is.
- I have checked the image information, the channel is recognized and the channel order is correct.
- I have also updated bio-formats to the latest version.
Though I can change the colour of each channel with channel tools, it is still big trouble because I have to change the color manually based on the information.
Can anyone help me please? Thanks.