I am a relatively new user of imagej and have the following issue:
I’d like to use color thresholding to calculate the % area of green leaves compared to the background.
My process is:
Adjust → Color threshold
Hue: 39, 109 (Pass unchecked)
Sat: 0, 255 (Pass checked)
Brightness: 0, 255 (Pass checked)
Default, Red, HSB, Dark Background checked
Image type → 8-bit color
Analyze → Measure (with area % selected)
With “Bi 1D”, this process gives 38% which is real. With “Bi 2F” and other images of this same batch, it gives 100%. Can someone please figure out how to fix this?
Thank you so much in advance.