I am trying to get ImageJ to count the number of branch points in the attached image. I successfully skeletonize with Skeletonize (2D/3D), but when I run Analyze Skeleton (2D/3D) it gives me numbers that are incorrect. I’ve attached a picture of my settings, and results.
According to the program I have 438 junctions (I counted 262), 260 endpoint voxels (I counted around 60), 48 junction voxels (makes no sense if I have hundreds of junctions), 30.04 triple points (makes no sense why this is not an integer), and 217 quadruple points (which would not add up with the triple points to make the correct number of junctions). None of these numbers make sense.
Can someone help? What is the reason behind these numbers? Does it have to do with the loops in the skeleton?
Thank you so much,