Hello CellProfiler team and users,
We are interested in studying some microtubule binding proteins, which, when unperturbed, localize on microtubules and form filamentous structures in cells. Our goal is to find conditions that perturb the interaction between these proteins and microtubules.
To do so, I have built a pipeline to identify filament-like structures in cells and calculate the ratio of area occupied by filament-like structures in each cell. The current version I have kind of does what I want, but it does’t seem to be particularly sensitive, and it seems to be biased toward picking up brighter areas as “filament-like” regardless how the images actually look like. Hope I will be able to learn some tips so I can improve my pipeline. Thanks a lot in advance!
MK014_v1_1.cpproj (87.8 KB)