I am the first time user to cell profiler. I want to do 3d segment of the attached image.
I followed the pipeline from here but not getting the correct output.
After watershed algorithm it is telling me that my segmented image has 3,35,140 cells. This is wrong because each cell get segments in multiple pieces not as a whole.
This is screenshot output of Rescale filter and watershed filter.
I am sharing the a small piece or original tif data and the pipeline what I made to do the segmentation.
If anyone know how to improve the pipeline please help me. Thank you.
Here is the link of input data and the pipeline