I’m trying to convince my boss that using the cell profiler program is as good as using my hours in manual colonies counting!
this is the the pipeline I created using the red colonies yeast cp.
I’m starting with the original picture that I crop using photoshop, making 7.tif
then I run this picture using the pipeline.
the thing is that using the program the colonies number is quite bigger that the result i get whit manual counter. I guess it’s due to the program counting many colonies at the border where there are no colonies.
I tried to crop the picture trying to avoid the light shadows and the glares, but I can’t cut in a way that there are no colonies at the borders.
can you help me with that please!!
yeast colony counter.cp (9.63 KB)