Applications where the relative location of one image to other images matter are becoming more abundant. I think this is partly due to more and more “intelligent microscopy” approaches where people take large low-res overview images and then (automatically) several smaller high-res images inside of the low-res images (this is done both in LM as well as in EM). I think another reason is that we realise that viewers like BigDataViewer in fact allow us to put all those images on top of each other and see the high-res images in the context of the low-res image, which is awesome. In addition, any kind of tiled image data that needs to stitched is another source of data where relative locations matter.
I was thus wondering whether it would be something of general interest to improve bio-formats in terms of the metadata that specifies the relative location of images?
For example, @NicoKiaru spotted the following issues with the current implementations:
- is the position specified by bioformats the center or corner ?
- sometimes the stage/sample can be flipped in XY, or in Z
- sometimes BioFormats does not know the unit.
I don’t know, but maybe, if enough people are interested one could try to write an application to get some funding for someone to work on this?
I cc some people that could be interested in this.