I am counting cells in multiple channels (brightfield and RFP) using IdentifyPrimaryObjects for each channel and combining them into a single, larger object set using CombineObjects for further classification (live vs. dead). It seems that the CombineObjects module does not add all unique objects from the “object set to combine” to the “initial object set”–only some objects are added instead of all unique objects.
In the attached pipeline, the CombineObjects #22 does not merge ALL unique Brightfield_Filtered objects into the DeadCells objects. Some objects are merged but not others and I’m not sure what is causing this issue. Could you provide any insight into why this is occuring? I am a newer user and it’s possible I’m using the module incorrectly. I haven’t found any other topics reporting this issue.
Thank you for your time,