I’m having an issue with opening some .nd2 files in Fiji. When I import them, the image shows a weird striped pattern where every channel is shown as separate horizontal lines that have the lines from other channels in-between them.
You can see the pattern appearing in this zoom-in. It should be a 5 channel composite (4 fluorescent + brightfield), but here the channels appear as lines. When only looking at one channel, the other lines disappear and there are 4 black lines instead.
These images open as they should in NIS-Elements viewer. Moreover, during the same imaging session I acquired other images that open up in Fiji without any problems. The only differences are that they have only 2 channels and more tiles.
I updated my Fiji to the latest version (v1.51o) and also tried to open the file on another computer, but that didn’t help. I also tried different Bio-Formats importing options but couldn’t find anything that would have resolved the issue.
I would greatly appreciate any help or suggestions you could give.
You can find an image that gives this issue here: https://www.dropbox.com/s/6s4asb46mpduf12/sample_image.nd2?dl=0