I’m trying to import multiple MP4 files from a microscope camera. The video files are shot at 60 fps with 1080p resolution. When I import and save these videos in FIJI using the ffmpeg plugin, the resulting file size gets prohibitively large and the software hangs up (I’m saving them in TIFF or AVI). Here’s the Dropbox link to one of the MP4 files.
The video shows a dendrite growing between two pointed electrodes, I want to calculate the area of dendrite in each frame to quantify the areal growth of the dendrite with time.
How do I go about importing the files? For the analysis, I’m planning to crop the video to only have the strip of electrolyte where the dendrite is growing and then use thresholding to select the dendrite area in each frame. If you have a better idea about how to analyse the video to get dendrite area with time, I’d appreciate that as well.