Importing and Using TrackMate



I imported an mp4 video file into Fiji to use the TrackMate function. When I attempt to open TrackMate, it says my file is “RGB: invalid”, and won’t let me go to the next step to actually run the program. Is there a way that I can fix this issue, maybe by converting to grayscale somehow? My video is in RGB color (though the video itself is black and white), so the program won’t let me change the format to 8-bit, 16-bit, etc.

Any ideas?


Yes, TrackMate requires grayscale input.

Why wouldn’t it let you? Is there any error message when you run Image > Type > 8-bit?