I was wondering if anyone had successfully imported ROI from .nd2 files into FIJI. I have a user whose FRAP data was captured in NIS-Elements (Nikon). The .nd2 files, when opened in Elements, have the ROIs so I think they must be stored in the metadata somewhere but I can’t find any information about them.
This user has many images they want to analyse in FIJI now and I’m hoping to find an alternative to them opening every image in Elements and saving the ROIs for each one to use going forward.
An example .nd2 file should be available at this link: