When importing a .lif file containing Leica FLIM data, the /FLIM/FlimDecayTime channel displays pixel values that do not correspond to the calculated lifetimes. There does not seem to be a linear relationship between the pixel values and the lifetime. Is there any conversion necessary or possible?
e.g. For a 2-component FLIM dye, in the Leica LASX software the calculated and separately saved lifetimes range between 0.05-0.836ns for lifetime 1 and between 3.195-6.373ns for lifetime 2. Saving the data as .tif in LASX gives me pixel values of 50-836 and 3195-6373, respectively.
However, when opening the /FLIM/FlimDecayTime 1 and 2 channel from a .lif file in ImageJ, it gives me pixel values of 13529-15073 and 17278-17917, respectively.
The example .tif files and the .lif project can be found here:
(Relevant files in the .lif project are "20200601_HEK_02.lif - 00_0nN_0mbar/FLIM/FlimDecayTime 1/2 ch1 - C=0)
Thanks a lot for your help,