I am using the BigStitcher plugin for time-registering my data set, which is an explant from embryos, where nuclei have been imaged.
I first detected interest points in these images based on nuclei and registered first using Fast Descriptor-based (translation invariant) algorithm followed by another round of registration with the Assign Closest Point with ICP (with no invariance) algorithm.
The registration itself works great. However, after registration the entire image shrunk dramatically. Any ideas why and how to solve this problem? I am a bit clueless here.
You can download both the non-registered xml and the registered xml files here (https://www.dropbox.com/sh/bstsnddx12pmlyu/AAA7AX_X3rCxAyokp1gwjQjOa?dl=0) and you will see the difference.
Thanks in advance for the help.