I am trying to do analysis on the 2D spatial distribution of intensity values on a microscope image. The area I am analysing has an irregular curvature so I used the straighten tool and then extracted the pixel values. But I am concerned that the straightening has distorted the intensity distribution.
Can anyone provide some explanation on if the straighten tool will alter the intensity values? I saw that the straighten function performs some interpolation but I don’t understand what is actually happening.
Thank you so much for any help!