When applying ImageJ’s awesome
Straighten... command in below image
run("Straighten...", "title=ClearedPlanula_Phalloidin_Hoechst-2.tif line=500 process");
There are regions where the data get significantly distorted (see second screenshot).
From just looking at the shape of the line ROI that should be straightened I somehow would not expect this. But maybe that really is the best transformation one can compute? I don’t have much experience with such operations and thus thought I ask here whether someone has an opinion or, even better, would know a way how to improve this.
Thank you so much for your time!
Image and line ROI: data.zip (1.4 MB)