ImageJ quantification

Good day. I am conducting semi-quantification of an agarose gel using ImageJ. However, I loaded only 2 uL for my DNA ladder while 5 uL for my sample. Is there any way I can do in order to use the data I obtained using this method?

Please explain more about what you have and what you want to do. Not all of us are biologists; or whatever field you are part of.