I have an image with a few small cells. My goal is to add them to the ROI manager, which I am able to do using the wand tool in ImageJ. My next step is to use a Euclidean Distance Transform to find the distance between the cells and a tissue outline that is in a different image. I can successfully do this.
However, my issue is this: when finding the distance, I don’t know if it is finding the distance between the tissue border and cells’ border, or if it is finding the distance between the tissue border and the cells’ centers.
Does anyone know of a way to find the center of an object in ImageJ and add it to the ROI Manager?
So far, I have tried the following: make a distance map of the image with the cells and find maxima. Is there anything I can do after that to add the point at the maximum to the ROI manager? Any help is greatly appreciated!