I am facing issues with setting the threshold values for images. In the attached image you can see that not all of the GFP is captured. But if I reduce the threshold value to include the projections, then the boundary for the other cells also is increased capturing void area. Is there any correction or step that can be included to correctly capture the approximate boundary of the cell?
Included the image below to highlight the clump of cells as well as the projections that haven’t been captured.
Please let me know if anyone has similar issues and how do you deal with it.
Thanks in Advance!
B10_01_1_3_DAPI_001.tiff (3.2 MB)