I have an issue with my images when I change the properties from pixels to microns. I have a 3D image of a red blood cell (stack of 96 slides), I open it with ImageJ and it automatically reads it in pixels (512x512). If I open the image with the 3D viewer WITHOUT changing its properties, I have a nice red blood cell! with the expected shape. But when I change the image properties to microns with the following values (as reported from the microscope manufacturer), [Pixel width: 0.182, Pixel height: 0.182, Voxel depth: 0.372] the image of my red blood cell gets distorted.
Would any of you be able to explain to me why this is the case and if this distortion can influence the values I then measure for volume and surface area using MorphoLibJ plugin and 3D manager?
I have attached two example images:
red blood cell with normal shape (unchanged properties)
red blood cell with distorted shape (much thicker than the other one)
I have been struggling with this issue for the last two days and cannot seem to find an answer anywhere.
Thank you in advance!