Good time of a day, friends.
We have faced a temporary (I HOPE) difficulties in our laboratory: our confocal microscope is broken.
So we are switching to fluorescent one, which is not really good.
Some examples of images (not best, not worst) are attached. (somewhy they are not loading dropbox link)
So I want to ask for advices:
What can we do on the software level to make them better in scientifical part. My guess is to take short time series and marge “average” image, but maybe you can give some other ideas. Or maybe tips how to do it better.
What can we do on software level to make them nice. Even if not scientifically correct - for some presentations. I perfectly know, that there are some processings, which will dramatically change “information”, and it is not appropriate to calculate data after such processing. But sometimes just beautifull image is needed.
Mainly noise and resolution are the problem. Should I invest some times in deconvolution? Or anything else? I am experimenting myself, but some tips would be nice. There are quite a lot of approaches and as always its hard to understand without help are you doing something wrong or you should use another approach.
Thank you a lot for your time.