Image J on fluorescence signals on chromosomes



I’m trying to quantify the fluorescence signals of chromosomes. I have my images took from my CCD and now I tried to quantify the signals. My question is if I use the value of integrated density it changes depending on the area that I selected. But if a signal is by chance bigger because the chromosome in that mitosis is bigger than the other one, I obtain a different value that is not related with the different intensity of the signal, but It is related more directly to the bigger area selected, being the IntDensity= area * mean gray value. If it’s correct, what other parameters i can use that is less relative?
thanks guys :slight_smile:


Hello @erin88 !!

Welcome to the Forum!

Perhaps reading through this older forum post will be helpful:

But if you can perhaps share with us an example image and the experimental question, describing what you wish to measure and how - we can better guide you in developing a robust analysis workflow.

eta :slight_smile: