I’m interested in Image Cytometry, and I have to study ploidy from sputum sample. I use Feulgen reaction to stain slides. To calculate ploidy status, I need Integrated Optical Density of cells in slide.
Is it possible to have this parameter, and also other parameters as area, optical density…, with Cell Profiler 2.0 (v.10415)?
Is it possible to have some pipelines for my experiment?
I attach same image for example.
Thank a lot