Illumination Correction

cp11 pipeline.cp (10.9 KB)




I needed to overexpose areas of my field in order to light the entire region, but now I am having difficulty correcting the lighting. The correction effectively blacks out the majority of the image. Is there a different way to subtract/divide the illumination correction?
Thanks

Hi,

You have the correct modules in place to do the correction, but the settings for the CorrectIlluminationCalculate modules are not quite optimal.

Try the following:

  • In CorrectIlluminationCalculate: Change the method from “Regular” to “Background”, set the block size to 50, no rescaling, set smoothing method to median, and the filter size calculation to ‘Manual’, with a size of 100 for both channels.
  • in CorrectIlluminationApply: Change the application method to “Subtract”.

This should get you closer, though your object identification settings probably need to be adjusted accordingly.

Regards,
-Mark