Illumination Correction of multiple images with loaded function




I was trying to do illumination correction of hundreds of images by using some illumination calibration images taken on fluorescent calibration slides. I was able to save and load the illumination correction function as a npy file following and However, when I tried to load images with the illumination function, only one image would be processed because “channels have different numbers of images”. Is there a way to process all the images at the same time without running batch processing? Thank you.


Yes! You need to match the illumination correction functions to the images to be corrected by some piece of Metadata- see the help for the “NamesAndTypes” module for examples.