Illumination Correction for differential protein expression among cells




I have three different population of cells in an image. Those cells are expected to express different amount of a particular protein. I would like to quantify the expression of protein in those cells using fluorophore conjugated antibody against that protein. In this case, as there will be differential fluorescence intensity among cells, what kind of illumination function should I use? Please help me to sort it out.

With regards,


What sort of illumination issues are you trying to correct for, and what are you trying to identify and measure?