Hi so I am using Ilastik, and have input the pre-processed (smoothed, resized, cropped) TIFF images that were pre-processed using CellProfiler.
When I input the TIFFs into the Ilastik ‘Input Data’ box the channels for each of my antibodies is missing.
Please note that yes, I did include correctly the panel.csv listing each antibody, with column names “Metal tags”, “group id”, and “ilastik”, as the experimental design.
How do I have the channel selection in Ilastik?
Should I be inputting HDF5s of my images instead of TIFFs?
Thank you very much.
Cell Profiler version 2.2.0