Beginner here! I am trying to quantify the area stained by CD68 in my slides. CD68 will stain macrophages and foreign body giant cells (FBGCs) which are going crazy trying to surround my constructs (white circles in the picutre, they disolve during paraffin embedding). My construct is around 100 µm thick and sometimes it is completely surrounded by FBGCs to the extent that it is not possible to know if it just one giant cell or several. Staining is with NovaRED and not DAB.
I have tried Fiji (manual color deconvolution, then thresholding and measuring the surface area), I also tried the WETA segmentation tool. But I was wondering if there is a way to do it with QuPath in an automated way.
Can you help me? I am still going to take the images, so I can also do tiles, that’s why I am very interested in QuPath.
Kindly find attached some images in Google Drive of several time points. Thank you.