IHC Fluorescence Analysis


I am new to ImageJ and trying to do fluorescence analysis for seminiferous tubules in testis. Does anyone know how to quantify the fluorescence from the images? Also, I am trying to figure out how to analyze just the fluorescence of specific areas within the section without getting the whole section such as just the spermatogonia.

Thank you!!!

Good day!

You may have a look at this discussion:

And please be aware of the important warning given by Gabriel Landini:

Unfortunately IHC is not stoichiometric and so you won’t get a reliable
measure of how much product you have. Most stains are not “quantitative” (with
some exceptions like Feulgen and phalloidin for example). […]
What you see as dark has no accurate relation to the amount of antigen present, […]