I have three channels (A, B, C), each a different color. I’m interested in identifying all A objects that also contact/overlap with B and C, i.e., a triple comparison. I’ve detected objects separately on each channel and have no difficulty comparing two channels (e.g. A to B, B to C, etc) using the “relate objects” module but it’s not clear how I can compare all three channels. Mainly, I’d like to know of all the A objects that contact/overlap B, how many of these also contact/overlap C.
In the relate objects image window, it shows me all the A objects that contact B. If I could somehow rename this subset of objects and point to them, I could perhaps relate them to C.
Here is the biology if anyone is interested… A=postsynaptic protein (some contained within B), B= cell plasma membrane protein (outline of cell), C=presynaptic protein (some contacting A). I want to find the postsynaptic proteins (A) within a cell’s membrane (B), that receive presynaptic input (contact or overlap with C).
Any help would be much appreciated. I love this software but am a total newb!