I need to identify puncta and it works somewhat, but it misses puncta that are close to the background of the cell. Is there anyway to identify objects based on the intensity? Additionally, is there a way to identify areas that have a higher intensity than the surrounding background?
Current pipeline rundown:
- Gaussian Filter on image with cells and puncta
- Enhance features on image with cells and puncta
- Identify primary objects
- Filter out object that do not meet size or intensity requirements
Here’s the pipeline:
puncta.cpproj (450.5 KB)
Here’s the image:
Example: in this image the puncta wasn’t found due to it being too close to the background, is there anyway to identify based on relative intensity?