I am trying to identify focal adhesions, but my pipeline identifies way to many spots.
I have tried everything I could think of with help from the forum, but my problem is not solved yet. I think the main issue is that the contrast between my focal adhesion stain (paxillin) and the background is not really good, but still by eye I can distinguish the spots from the background.
I attached my project with an image that gives me a lot of trouble. Can anyone help me?
PAXtest(2018-03-04)3.cpproj (100.2 KB)