I am trying to come up with a method to count the number of germinated pollen grains as well as the total number of pollen grains (both germinated and non-germinated) present in the image.
My goal is to have Image J calculate these values which I can then use to calculate the percentage of viable pollen grains in each image.
I have had success in counting the round, non-germinated pollen grains using Process >> Find Edges, followed by Image >> Adjust >> Threshold and finally Analyze >> Analyze Particles.
While this works well on circular, non-germinated pollen, I am unsure how to count germinated pollen which has a large pollen tube protruding from the pollen grain.
Does Image J have a plugin that can detect abnormally shaped objects? Do I need more specialized software such as CellProfiler?
I have attached an image for reference.