I’ve been working on a project identifying conidia (spores) in photos of a wash of C. Heterostrophus under a 10x microscope with substantial success. My next task is to take a similar wash and use it to quantify the portion of conidia that have begun to germinate. This involves measuring a small strand that has begun to grow out of the end of each conidia (see photo) and comparing its dimensions to the size of the conidia.
I have attached the identified objects from my original pipeline. As you can see, there is no problem in recognizing the strands growing out of conidia. I was wondering if anyone has tips for differentiating the conidia from the new growth. As yet, I have not thought of a good way to do this.