Hi from Hiroshima.
I’m new to CellProfiler and need some help.
I at hiroshima university am working cell biology related research on mesothelioma cells.
I found some cell profiler pipelines for counting/ measuring DAPI stained cells.
I would like to similar pipeline to work in Giemsa stained cells
I want to identify, count, and measure (relative area) of the Giemsa stained cells on the white background of the images (nuclei are stained blue and cytoplasm pink to orange by eosin).
I have some questions:
Can color images be used? like the one I uploded here GEIMSA_01.JPG
Is it possible to exclude some stain dirt?
I really want to put your package to use as it would greatly facilitate our work in this and other studies. Would also like to become better acquainted with Cellprofiler in general.
Many thanks in advance,
VISHWA JEET AMATYA
Mesothelioma Pathology Research
Department of Pathology,