I am trying cell profiler 2.0 to analyze few tumor images. However the images are not similar to provided in the example images and pipelines.
I have some light microscopy images of the some brain tumor cell line. I’m trying to track their growth over time.
I have attached a sample image.
I converted image to grayscale and inverted with imagemath. Then I tried to use regular treshold method to identify the object and further wanted to quantify the parameters like area, shape etc.
Can I use cell profiler for objects like these??Please let me know! Waiting for your reply!