Hi, I am an undergraduate student using this software for my honors project.
I have some problems in identifying closely packed nuclei of stem cells. I have played around with the settings in primary object identification, have also increased the resolution of the picture. I understand that the software might not be able to identify closely packed nuclei. But I am trying my best to identify as much nuclei as I can to extract information from the different colors. Am currently using otsu global on three class settings (foreground), have changed the nuclei settings accordingly to the image as well.
Have attached the pipeline I am using and 2 images too
Thanks in advance!
Quantifying DAPI.cp (5.43 KB)