I am interested in identifying cells that have lines through them (our experiment involves irradiation in this pattern).
I have looked at a related pipeline of counting GFP+ cells however I am not able to adapt it to this purpose since
I am not sure how to find lines in Cell profiler.
I have attached the image with two channels–the first channel is DAPI (nuclei) and the second channel detects line
formation of a certain GFP-fused protein.