I’m trying to create a pipeline that will identify RFP+ cells and GFP+ cells. And I want to make sure from my DAPI channel that each identified cell has ONLY ONE NUCLEUS.
My current pipeline (uploaded here: PAX6-1.cpproj (830.7 KB)
) involves three IdentifyPrimaryObjects steps. One identifies RFP+ cells, one identifies GFP+ cells, and one identifies nuclei. And then two IdentifyTertiaryObjects modules relate nuclei to GFP+ and RFP+ cells.
However, I am wondering if there is a better way to make sure that I’m actually identifying only RFP and GFP+ cells with one nucleus…? I wonder if I should be using a FilterObjects or Relate Objects module instead?