I am working with confluent layers of human epithelial cells and am interested in characterizing the quality of their cell-cell junctions. I have several stained ZO-1 images and would like to identify these borders and have my pipeline output an intensity reading as a function of cell-edge distance. In other words, I would like to individually identify the borders around each cell and see how the intensity varies along that border. Is this possible to do with CellProfiler?
So far, my pipline (attached) uses the maskimage and correctilluminationcalc functions to identify my cell bodies and it (for the most part) correctly identifies the cells by their corresponding junctions. I am not sure what to do from here.
I’ve also attached an image of the ZO-1 staining.
Thank you so much for your help–it is really appreciated.
Cell_identify with masking .cp (8.17 KB)