I’m one of the new and excited users of CellProfiler.
But since a couple of days I’m struggling with the IdentifyPrimAutomatic-module and especially with the threshold correction factor and bounds.
Are there any handful hints and tricks to initialize these values? And what about the others?
Just playing around, many trial-and-errors did not lead to acceptable and stable results.
An example of one of the images that we have to quantify, mostly with the MeasureObjectAreaShape-module, can be found on
Any idea or method will be greatly appreciated. Thanks in advance for sharing!